Tree shrew is a novel and high-quality experimental animal model. In this study, the real-time polymerase chain reaction methods were established to detect infection-related cytokines interleukin-6 (IL-6), IL-8, IL-10, IL-17A, interferon-γ (IFN-γ) and housekeeping gene glyceraldehyde-phosphate dehydrogenase (GAPDH) of tree shrew. The results indicated that the establised methods had good specificity. The high point of the linear range of these reagents reached 1 × 1010 copies, and the low points ranged from 10 copies (IL-6, IL-17A), 100 copies (IL-10, GAPDH) to 1 000 copies (IL-8, IFN-γ). In this interval, the linear correlation coefficient R2 of each reagent was greater than 0.99. The lowest detectable values of IL-6, IL-8, IL-10, IL-17A, IFN-γ and GAPDH were 8, 8, 4, 8, 128 and 4 copies, respectively. The results showed that the established detection methods had good specificity, sensitivity and wide linear range. The methods were suitable for detection of multiple concentration range samples, and could be used for the subsequent studies of tree shrew cytokines.
Citation：
LI Xiao, LIU Wenkuan, QIU Shuyan, XU Duo, ZHOU Zhichao, TIAN Xingui, LI Chi, GU Shujun, ZHOU Rong. Construction of real-time polymerase chain reaction detection for infection-related cytokines of tree shrew. Journal of Biomedical Engineering, 2019, 36(3): 407-413. doi: 10.7507/1001-5515.201801036
Copy
Copyright © the editorial department of Journal of Biomedical Engineering of West China Medical Publisher. All rights reserved
| 1. |
王应祥. 中国树鼩的分类研究. 动物学研究, 1987, 8(3): 213-230.
|
| 2. |
徐新平, 陈红波, 贲昆龙. 树鼩在医学生物学中的应用. 中国实验动物学报, 2005, 13(3): 187-190.
|
| 3. |
Cao J, Yang E B, Su J J, et al. The tree shrews: adjuncts and alternatives to primates as models for biomedical research. J Med Primatol, 2003, 32(3): 123-130.
|
| 4. |
Panda S, Kumari L, Panda S. Structural analysis of CD59 of Chinese tree shrew: A new reference molecule for human immune system specific CD59 drug discovery. Current Drug Discovery Technologies, 2018, 15(4): 326-334.
|
| 5. |
徐林, 张云, 梁斌, 等. 实验动物树鼩和人类疾病的树鼩模型研究概述. 动物学研究, 2013, 34(2): 59-69.
|
| 6. |
Xiao J, Liu R, Chen C S. Tree shrew (Tupaia belangeri) as a novel laboratory disease animal model. Zoological Research, 2017, 38(3): 127-137.
|
| 7. |
Tsukiyama-Kohara K, Kohara M. Tupaia belangeri as an experimental animal model for viral infection. Exp Anim, 2014, 63(4): 367-374.
|
| 8. |
Yang Z F, Mok C K, Liu X Q, et al. Clinical, virological and immunological features from patients infected with re-emergent avian-origin human H7N9 influenza disease of varying severity in Guangdong province. PLoS One, 2015, 10(2): e0117846.
|
| 9. |
Chen Weiwei, Nie Weimin, Xu Wen, et al. Cross-sectional study of the relationship of peripheral blood cell profiles with severity of infection by adenovirus type 55. BMC Infect Dis, 2014, 14: 147.
|
| 10. |
Fan Yu, Huang Zhiyong, Cao Changchang, et al. Genome of the Chinese tree shrew. Nat Commun, 2013, 4: 1426.
|
- 1. 王应祥. 中国树鼩的分类研究. 动物学研究, 1987, 8(3): 213-230.
- 2. 徐新平, 陈红波, 贲昆龙. 树鼩在医学生物学中的应用. 中国实验动物学报, 2005, 13(3): 187-190.
- 3. Cao J, Yang E B, Su J J, et al. The tree shrews: adjuncts and alternatives to primates as models for biomedical research. J Med Primatol, 2003, 32(3): 123-130.
- 4. Panda S, Kumari L, Panda S. Structural analysis of CD59 of Chinese tree shrew: A new reference molecule for human immune system specific CD59 drug discovery. Current Drug Discovery Technologies, 2018, 15(4): 326-334.
- 5. 徐林, 张云, 梁斌, 等. 实验动物树鼩和人类疾病的树鼩模型研究概述. 动物学研究, 2013, 34(2): 59-69.
- 6. Xiao J, Liu R, Chen C S. Tree shrew (Tupaia belangeri) as a novel laboratory disease animal model. Zoological Research, 2017, 38(3): 127-137.
- 7. Tsukiyama-Kohara K, Kohara M. Tupaia belangeri as an experimental animal model for viral infection. Exp Anim, 2014, 63(4): 367-374.
- 8. Yang Z F, Mok C K, Liu X Q, et al. Clinical, virological and immunological features from patients infected with re-emergent avian-origin human H7N9 influenza disease of varying severity in Guangdong province. PLoS One, 2015, 10(2): e0117846.
- 9. Chen Weiwei, Nie Weimin, Xu Wen, et al. Cross-sectional study of the relationship of peripheral blood cell profiles with severity of infection by adenovirus type 55. BMC Infect Dis, 2014, 14: 147.
- 10. Fan Yu, Huang Zhiyong, Cao Changchang, et al. Genome of the Chinese tree shrew. Nat Commun, 2013, 4: 1426.
