Research progress on point-of-care testing of blood biochemical indexes based on microfluidic technology_Journal of Biomedical Engineering

Authors：

ZHANG Huaqing ¹ , HU Canjie ² , QI Pengjia ² , YU Zhanlu ² , CHEN Wei ³ ,  TONG Jijun ²

1. Department of Clinical Medical Engineering, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, P. R. China;
2. College of Information Science and Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, P. R. China;
3. Hangzhou Baiyi Health Technology Co., LTD., Hangzhou 311217, P. R. China;

Corresponding author：

Keywords：

Real-time detection; Microfluidic; Blood biochemical index; Self-sampling

DOI：

10.7507/1001-5515.202406061

Video：

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[Abstract] Blood biochemical indicators are an important basis for the diagnosis and treatment by doctors. The performance of related instruments, the qualification of operators, the storage method and time of blood samples and other factors will affect the accuracy of test results, which is difficult to meet the clinical needs of rapid detection and early screening of diseases. Point-of-care testing (POCT) is a new diagnostic technology with the characteristics of instant, portability, accuracy and efficiency. Microfluidic chips can provide an ideal experimental reaction platform for POCT. This paper summarizes the existing detection methods for common biochemical indicators such as blood glucose, lactic acid, uric acid, dopamine and cholesterol, and focuses on the application status of POCT based on microfluidic technology in blood biochemistry. It also summarizes the advantages and challenges of existing methods and prospects for development. The purpose of this paper is to provide relevant basis for breaking through the technical barriers of microfluidic and POCT product development in China.

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37.	Niu J, Sun S, Liu P F, et al. Copper-based nanozymes: properties and applications in biomedicine. Journal of Inorganic Materials, 2023, 38(5): 489-502.
38.	Le T N, Le X A, Tran T D, et al. Laccase-mimicking Mn-Cu hybrid nanoflowers for paper-based visual detection of phenolic neurotransmitters and rapid degradation of dyes. Journal of Nanobiotechnology, 2022, 20(1): 358.
39.	Okhokhonin A V, Stepanova M I, Svalova T S, et al. A new electrocatalytic system based on copper (II) chloride and magnetic molecularly imprinted polymer nanoparticles in 3D printed microfluidic flow cell for enzymeless and low-potential cholesterol detection. Journal of Electroanalytical Chemistry, 2022, 924: 116853.
40.	Sun X T, Zhang Y, Zheng D H, et al. Multitarget sensing of glucose and cholesterol based on janus hydrogel microparticles. Biosensors and Bioelectronics, 2017, 92: 81-86.

1. Qudus M S, Cui X, Tian M, et al. The prospective outcome of the monkeypox outbreak in 2022 andcharacterization of monkeypox disease immunobiology. Frontiers in Cellular and Infection Microbiology, 2023, 13: 1196699.
2. Rahimi F S, Afaghi S, Tarki F E, et al. The historical epidemiology of human monkeypox: a reviewof evidence from the 1970 emergence to the 2022 outbreak. Tohoku Journal of Experimental Medicine, 2022, 258(4): 243-255.
3. Chew E J C, Tan P H. Evolutionary changes in pathology and our understanding of disease. Pathobiology, 2023, 90(3): 209-218.
4. Ferro P, Boni R, Slart R H, et al. Imaging of endocarditis and cardiac device-related infections: an update. Seminars in Nuclear Medicine, 2023, 53(2): 184-198.
5. Kamel H, Ramirez-Arcos S, McDonald C, et al. The international experience of bacterial screen testing of platelet components with automated microbial detection systems: an update. Vox Sanguinis, 2022, 117(5): 647-655.
6. Chandra P. Personalized biosensors for point-of-care diagnostics: from bench to bedside applications. Nanotheranostics, 2023, 7(2): 210-215.
7. Chen L, Guo X, Sun X, et al. Porous structural microfluidic device for biomedical diagnosis: a review. Micromachines, 2023, 14(3): 547.
8. Wang L, Cheng Y, Gopalan S, et al. Review and perspective: gas separation and discrimination technologies for current gas sensors in environmental applications. ACS Sensors, 2023, 8(4): 1373-1390.
9. Aryal P, Hefner C, Martinez B, et al. Microfluidics in environmental analysis: advancements, challenges, and future prospects for rapid and efficient monitoring. Lab on a Chip, 2024, 24(5): 1175-1206.
10. Shi L, Li Y, Jia C, et al. An overview of fluorescent microfluidics into revealing the mystery of food safety analysis: mechanisms and recent applications. Trends in Food Science and Technology, 2023, 138: 100-115.
11. Liang M, Zhang G, Song J, et al. Paper-based microfluidic chips for food hazard factor detection: fabrication, modification, and application. Foods, 2023, 12(22): 4107.
12. Ibrahim O A, Navarro-Segarra M, Sadeghi P, et al. Microfluidics for electrochemical energy conversion. Chemical Reviews, 2022, 122(7): 7236-7266.
13. Kumari M, Gupta V, Kumar N, et al. Microfluidics-based nano biosensors for healthcare monitoring. Molecular Biotechnology, 2024, 66(3): 378-401.
14. Xie M, Zhan Z, Li Y, et al. Functional microfluidics: theory, microfabrication, and applications. International Journal of Extreme Manufacturing, 2024, 6(3): 32005.
15. Juang Y J, Chiu Y J. Fabrication of polymer microfluidics: an overview. Polymers, 2022, 14(10): 2028.
16. Chen L, Yu L, Liu Y, et al. Space-time-regulated imaging analyzer for smart coagulation diagnosis. Cell Reports Medicine, 2022, 3(10): 100765.
17. Chen L, Yu L, Chen M, et al. A microfluidic hemostatic diagnostics platform: harnessing coagulation-induced adaptive-bubble behavioral perception. Cell Reports Medicine, 2023, 4(11): 101252.
18. Cui Y, Zhao J, Li H. Chromogenic mechanisms of colorimetric sensors based on gold nanoparticles. Biosensors, 2023, 13(8): 801.
19. Police Patil A V, Chuang Y S, Li C, et al. Recent advances in electrochemical immunosensors with nanomaterial assistance for signal amplification. Biosensors, 2023, 13(1): 125.
20. Yadav A K, Chan J. Activity-based bioluminescence probes for in vivo sensing applications. Current Opinion in Chemical Biology, 2023, 74: 102310.
21. Wang L, Li B, Wang J, et al. A rotary multi-positioned cloth/paper hybrid microfluidic device for simultaneous fluorescence sensing of mercury and lead ions by using ion imprinted technologies. Journal of Hazardous Materials, 2022, 428: 128165.
22. Yang L, Zhang Z, Wang X, et al. A microfluidic PET-based electrochemical glucose sensor. Micromachines, 2022, 13(4): 552.
23. Chinnamani M V, Hanif A, Kannan P K, et al. Soft microfiber-based hollow microneedle array for stretchable microfluidic biosensing patch with negative pressure-driven sampling. Biosensors and Bioelectronics, 2023, 237: 115468.
24. Mohan B, Kumar S, Xi H, et al. Fabricated metal-organic frameworks (MOFs) as luminescent and electrochemical biosensors for cancer biomarkers detection. Biosensors and Bioelectronics, 2022, 197: 113738.
25. Wang D, Liang Y, Su Y, et al. Sensitivity enhancement of cloth-based closed bipolar electrochemiluminescence glucose sensor via electrode decoration with chitosan/multi-walled carbon nanotubes/graphene quantum dots-gold nanoparticles. Biosensors and Bioelectronics, 2019, 130: 55-64.
26. Lu L, Zhang H, Wang Y, et al. Dissolution-enhanced luminescence enhanced digital microfluidics immunoassay for sensitive and automated detection of H5N1. ACS Applied Materials & Interfaces, 2023, 15(5): 6526-6535.
27. Song C, Yang Y, Tu X, et al. A smartphone-based fluorescence microscope with hydraulically driven optofluidic lens for quantification of glucose. IEEE Sensors Journal, 2021, 21(2): 1229-1235.
28. Zubkovs V, Wang H X, Schuergers N, et al. Bioengineering a glucose oxidase nano sensor for near-infrared continuous glucose monitoring. Nanoscale Advances, 2022, 4(11): 2420-2427.
29. Zhang M, Yang X, Ren M, et al. Microfluidic microwave biosensor based on biomimetic materials for the quantitative detection of glucose. Scientific Reports, 2022, 12(1): 15961.
30. Dungchai W, Chailapakul O, Henry C S, et al. Electrochemical detection for paper-based microfluidics. Analytical Chemistry, 2009, 81(14): 5821-5826.
31. Wang D, Liu C, Liang Y, et al. A simple and sensitive paper-based bipolar electrochemiluminescence biosensor for detection of oxidase-substrate biomarkers in serum. Journal of the Electrochemical Society, 2018, 165(9): B361-B369.
32. Oh C, Park B, Sundaresan V, et al. Closed bipolar electrode-enabled electrochromic sensing of multiple metabolites in whole blood. ACS Sensors, 2023, 8(1): 270-279.
33. Pinheiro T, Marques A C, Carvalho P, et al. Paper microfluidics and tailored gold nanoparticles for nonenzymatic, colorimetric multiplex biomarker detection. ACS Applied Materials & Interfaces, 2021, 13(3): 3576-3590.
34. Zhou Y, Cui A, Xiang D, et al. Point-of-care testing of four chronic disease biomarkers in blood based on a low cost and low system complexity microfluidic chip with integrated oxygen-sensitive membrane. Sensors and Actuators B: Chemical, 2024, 398: 134734.
35. Nuh S, Numnuam A, Thavarungkul P, et al. A novel microfluidic-based OMC-PEDOT-PSS composite electrochemical sensor for continuous dopamine monitoring. Biosensors, 2022, 13(1): 68.
36. Wagh M D, Hyderkhan R, Kumar P S, et al. Integrated microfluidic device with MXene enhanced laser-induced graphene bioelectrode for sensitive and selective electroanalytical detection of dopamine. IEEE Sensors Journal, 2022, 22(14): 14620-14627.
37. Niu J, Sun S, Liu P F, et al. Copper-based nanozymes: properties and applications in biomedicine. Journal of Inorganic Materials, 2023, 38(5): 489-502.
38. Le T N, Le X A, Tran T D, et al. Laccase-mimicking Mn-Cu hybrid nanoflowers for paper-based visual detection of phenolic neurotransmitters and rapid degradation of dyes. Journal of Nanobiotechnology, 2022, 20(1): 358.
39. Okhokhonin A V, Stepanova M I, Svalova T S, et al. A new electrocatalytic system based on copper (II) chloride and magnetic molecularly imprinted polymer nanoparticles in 3D printed microfluidic flow cell for enzymeless and low-potential cholesterol detection. Journal of Electroanalytical Chemistry, 2022, 924: 116853.
40. Sun X T, Zhang Y, Zheng D H, et al. Multitarget sensing of glucose and cholesterol based on janus hydrogel microparticles. Biosensors and Bioelectronics, 2017, 92: 81-86.

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Latest ArticlesResearch progress on point-of-care testing of blood biochemical indexes based on microfluidic technology

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