目的 探讨反义ATM多核苷酸（AS-Lipo）作用于裸鼠移植瘤对喉鳞状细胞癌(Hep-2)放射治疗敏感性的影响。 方法 用克隆生存率来检测喉鳞状细胞癌放射治疗后的生存能力；用流式细胞仪来分析喉癌细胞的凋亡情况；用Tunel染色法检测各组瘤体中细胞凋亡情况。 结果 在同等剂量的放射治疗下，AS-Lipo组的生存分数最低。接受4 Gy放射治疗后，行流式细胞仪检测，结果发现AS-Lipo组的凋亡率为（30.7 ± 1.31）%，远高于转染Sen-Lipo、Mis-Lipo、Lipo组。ATM AS-Lipo+放疗组的细胞凋亡数显著高于单独放射治疗组；ATM AS-Lipo+放射治疗组裸鼠瘤体内的凋亡指数为（17.12 ± 4.2）%，与其他组比较差异有统计学意义。 结论 反义ATM多核苷酸增强了裸鼠喉鳞状细胞癌对放射治疗的敏感性。
Citation：
LI Li,FENG Jun,WANG Chaoli,JING Baoqian.. Effect of Down-regulated ATM Expression on Radiosensitivity for Laryngeal Squamous Cell Carcinoma in Nude Mice. West China Medical Journal, 2013, 28(5): 728-731. doi: 10.7507/1002-0179.20130226
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Copyright © the editorial department of West China Medical Journal of West China Medical Publisher. All rights reserved
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江继平, 冯俊, 唐嗣泉, 等．反义ATM在体外对喉鳞状细胞癌ATM蛋白表达影响的研究[J]. 川北医学院学报, 2011, 26(5): 394-396.
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李丽, 冯俊, 王朝莉, 等．反义ATM对喉鳞状细胞癌ATM mRNA、蛋白影响的体外研究[J]．川北医学院学报, 2012, 27(5): 438-440.
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- 1. Hristov B, Bajaj GK. Radiotherapeutic management of laryngeal carcinoma[J]. Otolaryngologic clinics of North America, 2008, 41(4): 715-740.
- 2. Li L, Story M, Legerski RJ. Cellular responses to ionizing radiation damage [J]. Int J Radiat Oncol Biol Phys, 2001, 49(4): 1157-1162.
- 3. Christopher WJ, Dariel PG, Cecile VRT. Aberrant recombination involving the granzyme locus occurs in Atm-/-T-cell lymphomas[J]. Human Molecular Genetics, 2005, 14(18): 2671-2684.
- 4. Helt CE, Cliby WA, Keng PC, et al. Ataxia telangiectasia mutated (ATM) and ATM and Rad3-related protein exhibit selective target specificities in response to different forms of DNA damage[J]. J BiolChem, 2005, 280(2): 1186-92.
- 5. Kastan MB, Lim DS. The many substrates and functions of ATM[J]. Nat RevMol Cell Biol, 2000, 1(2): 179-186.
- 6. Chong MJ, Murray MR, Gosink EC, et al. Atm and Bax cooperate in ionizing radiation induced apoptosis in the central nervous system[J]. Proc Natl Acad Sci USA., 2000, 97(2): 889-894.
- 7. Borges HL, Chao C, Xu Y, et al. Radiation-induced apoptosis in developing mouse retina exhibits dose-dependent requirement for ATM phosphorylation of p53[J]. Cell Death Differ, 2004, 11(5): 494-502.
- 8. 江继平, 冯俊, 唐嗣泉, 等．反义ATM在体外对喉鳞状细胞癌ATM蛋白表达影响的研究[J]. 川北医学院学报, 2011, 26(5): 394-396.
- 9. 李丽, 冯俊, 王朝莉, 等．反义ATM对喉鳞状细胞癌ATM mRNA、蛋白影响的体外研究[J]．川北医学院学报, 2012, 27(5): 438-440.
