目的 比较使用流式细胞仪355 nm和407 nm激光器激发Hochest33342检测细胞凋亡。 方法 通过ATO药物诱导急性早幼粒白血病细胞(NB4)及血清饥饿法诱导人肺癌细胞(NCl-H292)细胞凋亡,取24、48 h时间点收集细胞,进行Hoechst33342-碘化丙啶(PI)双染,分别在配置有两种激光器的流式细胞仪上检测细胞凋亡。 结果 细胞经处理后24 h,355 nm激光器检测NB4细胞凋亡率Hoechst33342+/PI-:(28.20 ± 4.80)%;NCl-H292细胞凋亡率Hoechst33342+/PI-:(22.47 ± 2.78)%。407 nm激光器检测NB4细胞凋亡率Hoechst33342+/PI-:(25.10 ± 6.19)%。NCl-H292细胞凋亡率Hoechst33342+/PI-:20.47 ± 1.46%。处理后48 h,355 nm激光器检测NB4细胞凋亡率Hoechst33342+/PI-:(33.60 ± 3.75)%。NCl-H292细胞凋亡率Hoechst33342+/PI-:(26.77 ± 1.16)%。407 nm激光器检测NB4细胞凋亡率Hoechst33342+/PI-:(29.47 ± 2.33)%。NCl-H292细胞凋亡率Hoechst33342+/PI-:(31.47 ± 3.05)%。两种细胞处理后比处理前凋亡率明显升高,但355 nm激光器与407 nm激光器检测的凋亡结果差异不明显(P>0.05)。 结论 407 nm激光器激发Hoechst33342可检测细胞凋亡。
Citation: LI Xue,WU Yalan,HUANG Xin,HUANG Qiaorong,MENG Wentong.. Comparison between 355 nm and 407 nm Laser Exciting Hochest 33342 in the Detection of Apoptosis. West China Medical Journal, 2013, 28(6): 875-878. doi: 10.7507/1002-0179.20130274 Copy
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