Objective
To prepare cationic Vancomycin hydrochloride multivesicular liposome (MVL) and to inspect its quality.
Methods
Cationic Vancomycin hydrochloride MVLs were prepared by double emulsion method, and the storing solution of Vancomycin was prepared. The analysis method of Vancomycin in vitro was established; the specificity, precision, and resorption rate were estimated. Reverse phase high performance liquid chromatography (RP-HPLC) was used to determine the concentration of Vancomycin, encapsulation efficiency, and release characteristics in vitro. The formulation and pharmaceutical process were optimized by single factor experiments and orthogonal experimental design with the factor of encapsulation efficiency as the criteria. The liposome morphology was observed by optical microscopy and transmission electron microscopy. The particle size and Zeta potential were determined by Malvern instrument. The stability was analyzed by dynamic analysis.
Results
An RP-HPLC method was established for the assay of Vancomycin. The analysis method was precise, simple, and reliable for the quality control of Vancomycin. Vancomycin hydrochloride MVLs were round and well-distributed. The average particle size and the encapsulation efficiency were 3.3 μm and 24.9%, respectively. Zeta potential was 24.53 mV, and 90.5% of Vancomycin hydrochloride was released after 264 hours in normal saline under 37℃. Cationic Vancomycin MVLs
were stored for 1 month at 4 ℃, which mantained good stability.
Conclusion
Cationic Vancomycin hydrochloride MVLs have
good appearance, high encapsulation efficiency, good stability, and significant sustained release properties.
Citation：
YANG Duming,XU Yongqing,LI Fubing,LIU Hua,HE Xiaoqing. PREPARATION OF CATIONIC VANCOMYCIN HYDROCHLORIDE MULTIVESICULAR LIPOSOMES AND ITS QUALITY. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(4): 443-448. doi: 10.7507/1002-1892.20130106
Copy
Copyright © the editorial department of Chinese Journal of Reparative and Reconstructive Surgery of West China Medical Publisher. All rights reserved
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肖超菊, 齐宪荣, 艾尼瓦尔, 等. 顺铂缓释多囊脂质体的制备和体外释放性能研究. 药学学报, 2003, 38(2): 133-137.
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马涛, 尚北城, 徐贵丽, 等. 盐酸万古霉素阳离子脂质体的制备及其性质研究. 广东药学院报, 2010, 26(6): 551-555.
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- 1. Sutherland IW. The biofilm matrix: an immobilized but dynamic microbial environment. Trends Microbiol, 2001, 9(5): 222-227.
- 2. Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science, 1999, 284(5418): 1318-1322.
- 3. Kim S, Turker MS, Chie Y, et al. Preparation of multivesicular liposomes. Biochim Biophys Acta, 1983, 728(3): 339-348.
- 4. Mantripragada S. A lipid based depot (DepoFoaml technology) for sustained release drug delivery. Prog Lipid Res, 2002, 41(5): 392-406.
- 5. Tamilvanan S, Venkateshan N, Ludwig A. The potential of lipid—and polymer-based drug delivery carriers for eradicating biofilm consortia on device-related nosoeomial infections. J Control Release, 2008, 128(1): 20-22.
- 6. Kim HJ, Gias M, Jones MN. The adsorption of cationic liposomes to Staphylococcus aureus biofilms. Colloids Surf A Physicochem Eng Asp, 1999, 149(1-3): 561-570.
- 7. Ye Q, Asheman J, Stevenson M, et al. DepoFoam technology a vehicle for controlled delivery of protein and peptide drugs. J Control Release, 2000, 64(1-3): 155-156.
- 8. Taylor G, Kellaway IW, Steven J. Drug entrapment and release from multilamellar and reverse phase evaporation liposomes. Int J Pharm, 1990, 58(1): 49-55.
- 9. New RRC. Influence of liposome characteristics on their properties and fate//Philippot JR, Schuber F. Liposomes as tools in basic research and industry. Boca Raton: CRC Press, 1995: 3-20.
- 10. 张淼, 于叶玲, 唐星. 阿糖胞苷多囊脂质体的制备及体外释放度考察. 沈阳药科大学学报, 2009, 26(1): 1-5.
- 11. 肖超菊, 齐宪荣, 艾尼瓦尔, 等. 顺铂缓释多囊脂质体的制备和体外释放性能研究. 药学学报, 2003, 38(2): 133-137.
- 12. 马涛, 尚北城, 徐贵丽, 等. 盐酸万古霉素阳离子脂质体的制备及其性质研究. 广东药学院报, 2010, 26(6): 551-555.
