Objective To explore the differential expression of Sirtuin1 (SIRT1) in type A aortic dissection at diverse ages.Methods The expression of SIRT1 and monocyte chemoattractant protein-1 (MCP-1) in aortic tissue of the patients with type A aortic dissection (an aortic dissection group) and coronary heart disease (a control group) from 2019 to 2020 in the First Hospital of China Medical University was analyzed. In each group, the patients were divided into 3 subgroups according to the age (a younger subgroup, <45 years; a middle age subgroup, 45-60 years; an elderly subgroup, >60 years). The quantitative real-time PCR, Western blotting and immunochemical stainning were used to detect the mRNA or protein expression of SIRT1 and MCP-1. Results A total of 60 patients were included in each group, including 79 males and 41 females. There were 20 patients in the yonger, middle age and elderly subgroups for the two groups, respectively. Compared with the control group, the expression of SIRT1 mRNA decreased in the aortic dissection group (the younger subgroup: 4.54±1.52 vs. 8.78±2.57; the middle age group: 2.70±1.50 vs. 5.74±1.07; the elderly group: 1.41±1.33 vs. 3.09±1.14, P<0.001). Meanwhile, SIRT1 mRNA in the aortic dissection group declined with age (P<0.01). Compared with the control group, SIRT1 protein expression decreased significantly in the aortic dissection group (the younger group: 0.64±0.18 vs. 1.18±0.47; the middle age group: 0.43±0.26 vs. 0.69±0.32; the elderly group: 0.31±0.24 vs. 0.45±0.29, P<0.01). The Western blotting results showed that the expression of SIRT1 protein in the aortic dissection group decreased with age (P<0.01). The MCP-1 protein expression of younger and middle age patients in the aortic dissection group was increased compared with that in the control group (the younger group: 0.65±0.27 vs. 0.38±0.22; the middle age group: 1.08±0.30 vs. 0.46±0.36, P<0.001). MCP-1 expression increased with age (P<0.01). The result of immunohistochemical staining for SIRT1 protein was similar to that of Western blotting.Conclusion The expression of SIRT1 decreases in patients with aortic dissection disease, and declines with age. SIRT1 may play an important role in the treatment and screening of type A aortic dissection.
Citation：
ZHANG Wen, GU Tianxiang. Study on differential expression of Sirtuin1 in type A aortic dissection pateints at diverse ages. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2023, 30(4): 622-626. doi: 10.7507/1007-4848.202103016
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Copyright © the editorial department of Chinese Journal of Clinical Thoracic and Cardiovascular Surgery of West China Medical Publisher. All rights reserved
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Cai WT, Guan P, Lin MX, et al. Sirt1 suppresses MCP-1 production during the intervertebral disc degeneration by inactivating AP-1 subunits c-Fos/c-Jun. Eur Rev Med Pharmacol Sci, 2020, 24(11): 5895-5904.
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- 1. Nienaber CA, Clough RE, Sakalihasan N, et al. Aortic dissection. Nat Rev Dis Primers, 2016, 2: 16053.
- 2. LeMaire SA, Russell L. Epidemiology of thoracic aortic dissection. Nat Rev Cardiol, 2011, 8(2): 103-113.
- 3. Kane AE, Sinclair DA. Sirtuins and NAD+ in the development and treatment of metabolic and cardiovascular diseases. Circ Res, 2018, 123(7): 868-885.
- 4. Chen HZ, Wang F, Gao P, et al. Age-associated Sirtuin 1 reduction in vascular smooth muscle links vascular senescence and inflammation to abdominal aortic aneurysm. Circ Res, 2016, 119(10): 1076-1088.
- 5. Bossone E, Eagle KA. Epidemiology and management of aortic disease: Aortic aneurysms and acute aortic syndromes. Nat Rev Cardiol, 2021, 18(5): 331-348.
- 6. Soni SK, Basu P, Singaravel M, et al. Sirtuins and the circadian clock interplay in cardioprotection: Focus on sirtuin 1. Cell Mol Life Sci, 2021, 78(6): 2503-2515.
- 7. Zhang J, Ren D, Fedorova J, et al. SIRT1/SIRT3 modulates redox homeostasis during ischemia/reperfusion in the aging heart. Antioxidants (Basel), 2020, 9(9): 858.
- 8. Xia L, Sun C, Zhu H, et al. Melatonin protects against thoracic aortic aneurysm and dissection through SIRT1-dependent regulation of oxidative stress and vascular smooth muscle cell loss. J Pineal Res, 2020, 69(1): e12661.
- 9. França CN, Izar MCO, Hortêncio MNS, et al. Monocyte subtypes and the CCR2 chemokine receptor in cardiovascular disease. Clin Sci (Lond), 2017, 131(12): 1215-1224.
- 10. Hui X, Zhang M, Gu P, et al. Adipocyte SIRT1 controls systemic insulin sensitivity by modulating macrophages in adipose tissue. EMBO Rep, 2017, 18(4): 645-657.
- 11. Cai WT, Guan P, Lin MX, et al. Sirt1 suppresses MCP-1 production during the intervertebral disc degeneration by inactivating AP-1 subunits c-Fos/c-Jun. Eur Rev Med Pharmacol Sci, 2020, 24(11): 5895-5904.
