目的:研究TRAIL对卵巢癌COC1/DDP细胞生长的影响，以及化疗药物DDP等对TRAIL受体（DR4、DR5）表达的影响，揭示TRAIL与COC1/DDP细胞顺铂耐药性的关系。方法:用MTT法检测不同浓度TRAIL蛋白和TRAIL与DDP联合用药对COC1/DDP细胞生长的影响，用RTPCR方法检测DDP对TRAIL受体（DR4、DR5）表达的影响。结果:①TRAIL蛋白对COC1/DDP细胞生长有抑制作用，且随着TRAIL蛋白浓度升高，细胞抑制率逐渐上升。②DDP（2.5μg/mL）对COC1/DDP细胞生长抑制作用较弱（抑制率为3.31%），DDP在加入TRAIL蛋白后对细胞生长抑制率显著升高（P lt;0.05）。③DDP使COC1/DDP细胞的DR5表达水平显著增强为正常对照组的3.54倍（P lt;0.001）。结论:TRAIL蛋白对COC1/DDP细胞生长有抑制作用，DDP与TRAIL联合使用COC1/DDP细胞生长抑制更明显，TRAIL可逆转COC1/DDP细胞对DDP的耐药性，耐药性的逆转可能与DDP导致TRAIL受体DR5水平增高促进了肿瘤细胞的凋亡有关。
Citation：
LI Chunmei,PENG Zhilan,YIN Rutie. Effect of TRAIL to Reverse the DDP resistance of Ovarian Carcinoma Cell Line COC1/DDP. West China Medical Journal, 2009, 24(1): 105-107. doi:
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- 4. Sprick MR，Weigand MA，Rieser E，et al.FADD/MORT1 and caspase8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2[J].Immunity，2000，12（6）:599-609..
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- 8. Arts HJ，de Jong S，Hollema H，et al.Chemotherapy induces death receptor 5 in epithelial ovarian carcinoma[J].Gynecol Oncol，2004，92（3）:794-800..
- 9. Tomek S，Horak P，Pribill I，et al.Resistance to TRAILinduced apoptosis in ovarian cancer cell lines is overcome by cotreatment with cytotoxic drugs[J].Gynecol Oncol，2004，94（1）:107-114..
