Objective To investigate the expression of protein gene product 9.5 (PGP9.5) in human gastric cancer, and to find out the relations between its expression and carcinogenesis, invasion and metastasis of gastric cancer.
Methods The expression of PGP9.5 was detected by immunohistochemistry (SP) and Western blot in 80 samples of gastric cancer and 8 samples of normal gastric tissues.
Results ①Of 80 gastric cancer specimens examined, 56 cases (70.0％) showed staining with PGP9.5 in most tumor cells, whereas no PGP9.5 expression was detected in normal epithelium, which was consistent with the results of Western blot. ②The results of immunohistochemistry revealed that there were significantly correlations between the expression of PGP9.5 and the depth of invasion, the degree of differentiation, and the occurrence of lymph node metastasis (P lt;0.05), respectively; yet, there were no relation between the expression of PGP9.5 and age, gender, histopathologic type and TNM stage (P gt;0.05).
Conclusion PGP9.5 may play an important role in the invasion and metastasis of gastric cancer, and the invasion of gastric cancer could be detected by PGP9.5, which may be a useful molecular marker.
Citation：
ZHANG Xian,ZHANG Xiaobing,TAO Kaixiong,WANG Guobin. Expression and Clinical Significance of Protein Gene Product 9.5 in Human Gastric Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(1): 76-79. doi:
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- 1. Otsuki T, Yata K, Takata-Tomokuni A, et al. Expression of protein gene product 9.5 (PGP9.5)/ubiquitin-C-terminal hydrolase 1 (UCHL-1) in human myeloma cells [Ｊ]. Br J Haematol， 2004; 127(3)∶292.
- 2. Mandelker DL, Yamashita K, Tokumaru Y, et al. PGP9.5 promoter methylation is an independent prognostic factor for esophageal squamous cell carcinoma [Ｊ]. Cancer Res， 2005; 65(11)∶4963.
- 3. Hayashi H, Ochiai T, Suzuki T, et al. Superiority of a new AJCC-TNM staging system for gastric carcinoma [Ｊ]. Surgery, 2003; 127(5)∶129.
- 4. Takano T, Miyauchi A, Matsuzuka F, et al. PGP9.5 mRNA could contribute to the molecular-based diagnosis of medullary thyroid carcinoma [Ｊ]. Eur J Cancer， 2004; 40(4)∶614.
- 5. Takase T, Hibi K, Yamazaki T, et al. PGP9.5 overexpression in esophageal squamous cell carcinoma [Ｊ]. Hepatogastroenterology， 2003; 50(53)∶1278.
- 6. Lee YM, Lee JY, Kim MJ, et al. Hypomethylation of the protein gene product 9.5 promoter region in gallbladder cancer and its relationship with clinicopathological features [Ｊ]. Cancer Sci， 2006; 97(11)∶1205.
- 7. Yamazaki T, Hibi K, Takase T, et al. PGP9.5 as a marker for invasive colorectal cancer [Ｊ]. Clin Cancer Res， 2002; 8(1)∶192.
- 8. Tezel E, Hibi K, Nagasaka T, et al. PGP9.5 as a prognostic factor in pancreatic cancer [Ｊ]. Clin Cancer Res， 2000; 6(12)∶4764.
- 9. Yamashita K, Park HL, Kim MS, et al. PGP9.5 methylation in diffuse-type gastric cancer [Ｊ]. Cancer Res， 2006; 66(7)∶3921.
