【Abstract】Objective To observe the bacteria in cholesterol stones by electronic microscope and to explore the role of bacteria in the stone formation.
Methods Twelve patients with cholelithiasis underwent operations (male 6, female 6, average age 54.6 years） with cholecystolithiasis 5, extrahepatic and intrahepatic bile duct stone 1, common bile duct stone combining with gallstone 6. The cholesterol stones were observed by electronic microscope.
Results There were bacterial structures in the cholesterol stones and cholesterol crystals.
Conclusion There are bacteria in the core and peripheral of cholesterol stones, which suggests that bacteria may play an initial role in the formation of cholesterol stones.
Citation：
YANG Yulong,TAN Wenxiang,LIU Xiaobei,LI Xuetuo.. Observation of Bacteria in Cholesterol Stones by Electronic Microscope. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(5): 447-449. doi:
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- 1. 张圣道，韩天权. 胆石病的流行病学 [A].见：黄志强主编. 当代胆道外科学 [M]. 第1版. 上海： 上海科学技术文献出版社， 1998∶ 249～260.
- 2. 赵小团, 张圣道,付培彬.转铁蛋白对胆汁胆固醇成核过程的影响 [J].中华消化杂志, 1991; 11(4)∶93.
- 3. 方驰华，杨继震.胆囊结石需氧菌和厌氧菌分子生物学研究及意义 [J].第四军医大学学报， 1998； 19(6)∶681.
- 4. Maki T. Pathogenesis of calcium bilirubinate gallstone: role of E. coli, betaglucuronidase and coagulation by inorganic ions, polyelectrolytes and agitation [Ｊ]. Ann Surg， 1966; 164(1)∶90.
- 5. Jan YY, Chen MF. Percutaneous transhepatic cholangioscopic lithotomy for hepatolithiasis: longterm results [Ｊ]. Gastrointest Endosc， 1995; 42(1)∶1.
- 6. Swidsinski A, Ludwig W, Pahlig H, et al. Molecular genetic evidence of bacterial colonization of cholesterol gallstones [Ｊ]. Gastroenterology, 1995; 108(3)∶860.
- 7. Kawai M, Iwahashi M, Uchiyama K, et al. Grampositive cocci are associated with the formation of completely pure cholesterol stones [Ｊ]. Am J Gastroenterol, 2002; 97(1)∶83.
- 8. Lee DK, Tarr PI, Haigh WG, et al. Bacterial DNA in mixed cholesterol gallstones [Ｊ]. Am J Gastroenterol, 1999; 94(12)∶3502.
- 9. Cariati A, Cetta F. Re： Kawai et al. Bacteria are not important in the formation of pure cholesterol stones [Ｊ]. Am J Gastroenterol, 2002; 97(11)∶2921.
- 10. Kawai M, Iwahashi M, Uchiyama K, et al. Response to bacteria are not important in the formation of pure cholesterol stones [Ｊ]. Am J Gastroenterol, 2002; 97(11)∶2922.
