Objective To elucidate the etiology of DNA impairment of type Ⅱ alveolar epithelial cells(AT-II) of the rats fed with low selenium and high cadmium fodder,and the effect of Vitamin C.Methods With single cell gel electrophoresis technique,we observed the joint action of selenium,cadmium and vitamin C on DNA damage in AT-II cells of the eight groups of rats fed separately with:normal (2 groups),high Cd,high Cd+high VC,low Se+high Cd,low Se+high Cd+high VC,high Se+high Cd and high Se+high Cd+high VC fodder for 14 weeks.Results Compared with the control,there was no DNA changes have been observed in the high Se+high Cd+high VC group.However,in the high Se+high Cd group and high Cd+high VC group,DNA damage of AT-II cells can be detected clearly;in the low Se+high Cd+high VC group and high Cd group,the degree of the DNA damage is more serious than the above two groups;in the low Se+high Cd group,the extent of the DNA damage was the most serious on all of the groups be studied.Conclusion It is suggested that Se deficiency and simultaneously Cd overabundance may damaged DNA of AT-II cells of the rats significantly,however,Vitamin C may protect AT-II against the injury effectively.
Citation:
CAO Wenhua ,TIAN Hong,CHEN Ran,LI Jinguo,ZHOU Xin,WNAG Qingbao.. Effects of vitamin C on the DNA of type II alveolar epithelial cells of the rats fed with low selenium and high cadmium fodder. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(2): 132-134. doi:
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黎友伦,郑海涛,罗永艾.硒对呼吸系统感染的防治作用.广东微量元素科学,2004,11:1-5.
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Frisk P,Yaqob A,Lindh U.Indications of lenium protection against cadmium toxicity in cultured K-562 cells.Sci Total Environ,2002,296:189-193.
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相有章,边建朝,杨晓霞,等.铬对克山病病区粮喂养大鼠的甲状腺激素及cAMP水平的影响.中国地方病学杂志,1997,16:149-151.
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薛云,陈凡,陈焕春,等.猪肺Ⅱ型上皮细胞的分离和培养研究进展.动物医学进展,2005,26:5-7.
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田素增,谢敏,刘涛,等.慢性阻塞性肺疾病大鼠Ⅱ型肺泡上皮细胞凋亡水平的变化及吸入糖皮质激素对其的影响.中国呼吸与危重监护杂志,2007,6:381-383.
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Singh NP,McCoy MT,Tice RR,et al.A simple technique for quantitation of low levels of DNA damage in individual cells.Exp Cell Res,1988,175:184-186.
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10. |
刘德坚,马忠元,梁敬棠.镉对大鼠肾细胞毒性和脂质过氧化作用的影响,中国热带医学,2007,10:1755-1756.
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11. |
薛美兰,张秀珍,韩秀霞,等.大剂量维生素C对DNA氧化烷化损伤影响,中国公共卫生2006,2:177-179.
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12. |
刘 艳,张世蘋,蔡云清.硒对镉诱导肾细胞凋亡及其相关蛋白bcl-2、p53表达的影响,南京医科大学学报,2007,3:224-226.
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余日安.镉与DNA损伤、癌基因表达、细胞凋亡.国外医学 卫生学分册,2000,27:359-363.
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- 1. 黎友伦,郑海涛,罗永艾.硒对呼吸系统感染的防治作用.广东微量元素科学,2004,11:1-5.
- 2. 相有章,赵力军,曹恒常,等.克山病心肌炎扩张型心肌病血清、尿、发中化学元素水平测定.中国地方病学杂志,1988,7:167-169.
- 3. 邵世和,孙丽媛.微量元素硒与肿瘤的关系.北华大学学报,2003,4:223-226.
- 4. 彭双清,王军波,刘世杰,等.亚硒酸钠与硒代蛋氨酸对镉在大鼠体内分布的影响.中国预防医学杂志,2002,3:255-258.
- 5. Frisk P,Yaqob A,Lindh U.Indications of lenium protection against cadmium toxicity in cultured K-562 cells.Sci Total Environ,2002,296:189-193.
- 6. 相有章,边建朝,杨晓霞,等.铬对克山病病区粮喂养大鼠的甲状腺激素及cAMP水平的影响.中国地方病学杂志,1997,16:149-151.
- 7. 薛云,陈凡,陈焕春,等.猪肺Ⅱ型上皮细胞的分离和培养研究进展.动物医学进展,2005,26:5-7.
- 8. 田素增,谢敏,刘涛,等.慢性阻塞性肺疾病大鼠Ⅱ型肺泡上皮细胞凋亡水平的变化及吸入糖皮质激素对其的影响.中国呼吸与危重监护杂志,2007,6:381-383.
- 9. Singh NP,McCoy MT,Tice RR,et al.A simple technique for quantitation of low levels of DNA damage in individual cells.Exp Cell Res,1988,175:184-186.
- 10. 刘德坚,马忠元,梁敬棠.镉对大鼠肾细胞毒性和脂质过氧化作用的影响,中国热带医学,2007,10:1755-1756.
- 11. 薛美兰,张秀珍,韩秀霞,等.大剂量维生素C对DNA氧化烷化损伤影响,中国公共卫生2006,2:177-179.
- 12. 刘 艳,张世蘋,蔡云清.硒对镉诱导肾细胞凋亡及其相关蛋白bcl-2、p53表达的影响,南京医科大学学报,2007,3:224-226.
- 13. 余日安.镉与DNA损伤、癌基因表达、细胞凋亡.国外医学 卫生学分册,2000,27:359-363.