Significance of mRNA Expression of PARP-1 in Severe Acute Pancreatitis Associated Renal Injury in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

惠远见 , 王卫星 , 余佳 , 邓文宏 , 陈晓燕 , 崔周军 , 阿布力克木阿布力孜

Corresponding author：

Keywords：

急性胰腺炎; 肾损伤; 聚腺苷二磷酸核糖聚合酶-1; 机理

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的探讨聚腺苷二磷酸核糖聚合酶-1（poly ADPribose polymerase-1,PARP-1） mRNA在重症急性胰腺炎(severe acute pancreatitis，SAP)大鼠肾脏中的表达及意义。
方法 48只Wistar大鼠按随机数字表法分为SAP组和假手术组(SO)组，分别于造模术后1、3、6及12 h测定血清肌酐，观察胰腺和肾脏组织病理变化，并以RT-PCR法检测PARP-1 mRNA在肾脏中的表达水平。
结果 SAP组大鼠术后血清肌酐逐渐升高，于3、6及12 h明显高于SO组(P lt;0.05)。SAP组大鼠术后胰腺出现腺体破坏、腺泡坏死、出血、炎性细胞浸润等病理损害，且呈进行性加重； SO组各时相胰腺组织基本正常。SAP组大鼠术后出现肾小管上皮细胞变性、坏死、肾小球瘀血、缺血等改变，并随时间延长逐渐加重，其损伤程度在3、6及12 h明显较SO组严重(P lt;0.05)。SO组大鼠肾脏组织仅表达少量PARP-1 mRNA，而SAP组大鼠随病程延长肾脏组织中PARP-1 mRNA表达逐渐增加，自3 h时起明显高于SO组（P lt;0.01）。
结论在SAP发病过程中，PARP-1 mRNA的表达在肾脏组织中逐渐增加，PARP-1可能参与了SAP相关肾损伤过程。

Citation： 惠远见,王卫星,余佳,邓文宏,陈晓燕,崔周军,阿布力克木阿布力孜. Significance of mRNA Expression of PARP-1 in Severe Acute Pancreatitis Associated Renal Injury in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(3): 272-274. doi: Copy

1.	庄永敬, 于永扬, 吴桂荣. 乌司他丁对重症急性胰腺炎大鼠肾脏细胞凋亡及 bcl2 基因表达的影响 [J]. 中国普外基础与临床杂志, 2007; 14(6): 656659, 664.
2.	Pupelis G. Renal failure in acute pancreatitis. Timing of dialysis and surgery [J]. Przegl Lek， 2000; 57 Suppl 5: 2931.
3.	Kes P, Vucicevic＇ Z, Ratkovic＇ Gusic＇ I, et al. Acute renal failure complicating severe acute pancreatitis [J]. Ren Fail, 1996; 18(4): 621628.
4.	Szabó G, Bhrle S, Stumpf N, et al. Poly (ADPRibose) polymerase inhibition reduces reperfusion injury after heart transplantation [J]. Circ Res, 2002; 90(1): 100106.
5.	雷文章, 韦靖江, 沈文律, 等. 实验性坏死性胰腺炎多器官损害与内毒素血症的关系 [J]. 中华实验外科杂志, 1995; 12(3): 131132.
6.	Schmidt C, Hcherl K, Schweda F, et al. Regulation of renal sodium transporters during severe inflammation [J]. J Am Soc Nephrol, 2007; 18(4): 10721083.
7.	Kusske AM, Rongione AJ, Reber HA. Cytokines and acute pancreatitis [J]. Gastroenterology, 1996; 110(2): 639642.
8.	Yekebas EF, Strate T, Zolmajd S, et al. Impact of different modalities of continuous venovenous hemofiltration on sepsisinduced alterations in experimental pancreatitis [J]. Kidney Int, 2002; 62(5): 18061818.
9.	Dobosz M, Mionskowska L, Hac S, et al. Heparin improves organ microcirculatory disturbances in caeruleininduced acute pancreatitis in rats [J]. World J Gastroenterol, 2004; 10(17): 25532556.
10.	王云帆, 陈瑞芬. 急性胰腺炎肾脏损害的发生机制 [J]. 首都医科大学学报, 2004； 25(2): 276278.
11.	Zhang XP, Wang L, Zhou YF. The pathogenic mechanism of severe acute pancreatitis complicated with renal injury: a review of current knowledge [J]. Dig Dis Sci, 2008; 53(2): 297306.
12.	翟文龙, 张水军, 崔雪艳, 等. 雷公藤内脂醇对大鼠重症急性胰腺炎合并肾损伤防护作用 [J]. 中华肝胆外科杂志, 2006; 12(2): 138139.
13.	Amé JC, Spenlehauer C, de Murcia G. The PARP superfamily [J]. Bioessays, 2004; 26(8): 882893.
14.	Graziani G, Battaini F, Zhang J. PARP1 inhibition to treat cancer, ischemia, inflammation [J]. Pharmacol Res, 2005; 52(1): 14.
15.	Decker P, Muller S. Modulating poly (ADPribose) polymerase activity: potential for the prevention and therapy of pathogenic situations involving DNA damage and oxidative stress [J]. Curr Pharm Biotechnol, 2002; 3(3): 275283.
16.	Mazzon E, Genovese T, Di Paola R, et al. Effects of 3aminobenzamide, an inhibitor of poly (ADPribose) polymerase, in a mouse model of acute pancreatitis induced by cerulein [J]. Eur J Pharmacol, 2006; 549(13): 149156.
17.	Du L, Zhang X, Han YY, et al. Intramitochondrial poly(ADPribosylation) contributes to NAD+ depletion and cell death induced by oxidative stress [J]. J Biol Chem, 2003; 278(20): 1842618433.
18.	Pandol SJ, Saluja AK, Imrie CW, et al. Acute pancreatitis: bench to the bedside [J]. Gastroenterology, 2007; 133(3): 1056.e11056.e25.
19.	Gukovskaya AS, Pandol SJ. Cell death pathways in pancreatitis and pancreatic cancer [J]. Pancreatology, 2004; 4(6): 567586.
20.	Nakajima H, Nagaso H, Kakui N, et al. Critical role of the automodification of poly (ADPribose) polymerase1 in nuclear factorkappaBdependent gene expression in primary cultured mouse glial cells [J]. J Biol Chem, 2004; 279(41): 4277442786.
21.	Szabó C, Ischiropoulos H, Radi R. Peroxynitrite: biochemistry, pathophysiology and development of therapeutics [J]. Nat Rev Drug Discov, 2007; 6(8): 662680.
22.	Pacher P, Szabo C. Role of the peroxynitritepoly (ADPribose) polymerase pathway in human disease [J]. Am J Pathol, 2008; 173(1): 213.
23.	Mota RA, SánchezBueno F, Saenz L, et al. Inhibition of poly (ADPribose) polymerase attenuates the severity of acute pancreatitis and associated lung injury [J]. Lab Invest, 2005; 85(10): 12501262.
24.	Ku Y, Sharif R, Dhaulakhandi D, et al. Saluja A.Inhibition of poly (ADP ribose) polymerase attenuates the severity of caeruleininduced pancreatitis and associated lung injury by inhibition of NFkB activation [J]. Pancreas, 2003; 27(4): 392.
25.	Hashimoto K, Ethridge RT, Saito H, et al. The PPARgamma ligand, 15dPGJ2, attenuates the severity of ceruleininduced acute pancreatitis [J]. Pancreas, 2003; 27(1): 5866.

1. 庄永敬, 于永扬, 吴桂荣. 乌司他丁对重症急性胰腺炎大鼠肾脏细胞凋亡及 bcl2 基因表达的影响 [J]. 中国普外基础与临床杂志, 2007; 14(6): 656659, 664.
2. Pupelis G. Renal failure in acute pancreatitis. Timing of dialysis and surgery [J]. Przegl Lek， 2000; 57 Suppl 5: 2931.
3. Kes P, Vucicevic＇ Z, Ratkovic＇ Gusic＇ I, et al. Acute renal failure complicating severe acute pancreatitis [J]. Ren Fail, 1996; 18(4): 621628.
4. Szabó G, Bhrle S, Stumpf N, et al. Poly (ADPRibose) polymerase inhibition reduces reperfusion injury after heart transplantation [J]. Circ Res, 2002; 90(1): 100106.
5. 雷文章, 韦靖江, 沈文律, 等. 实验性坏死性胰腺炎多器官损害与内毒素血症的关系 [J]. 中华实验外科杂志, 1995; 12(3): 131132.
6. Schmidt C, Hcherl K, Schweda F, et al. Regulation of renal sodium transporters during severe inflammation [J]. J Am Soc Nephrol, 2007; 18(4): 10721083.
7. Kusske AM, Rongione AJ, Reber HA. Cytokines and acute pancreatitis [J]. Gastroenterology, 1996; 110(2): 639642.
8. Yekebas EF, Strate T, Zolmajd S, et al. Impact of different modalities of continuous venovenous hemofiltration on sepsisinduced alterations in experimental pancreatitis [J]. Kidney Int, 2002; 62(5): 18061818.
9. Dobosz M, Mionskowska L, Hac S, et al. Heparin improves organ microcirculatory disturbances in caeruleininduced acute pancreatitis in rats [J]. World J Gastroenterol, 2004; 10(17): 25532556.
10. 王云帆, 陈瑞芬. 急性胰腺炎肾脏损害的发生机制 [J]. 首都医科大学学报, 2004； 25(2): 276278.
11. Zhang XP, Wang L, Zhou YF. The pathogenic mechanism of severe acute pancreatitis complicated with renal injury: a review of current knowledge [J]. Dig Dis Sci, 2008; 53(2): 297306.
12. 翟文龙, 张水军, 崔雪艳, 等. 雷公藤内脂醇对大鼠重症急性胰腺炎合并肾损伤防护作用 [J]. 中华肝胆外科杂志, 2006; 12(2): 138139.
13. Amé JC, Spenlehauer C, de Murcia G. The PARP superfamily [J]. Bioessays, 2004; 26(8): 882893.
14. Graziani G, Battaini F, Zhang J. PARP1 inhibition to treat cancer, ischemia, inflammation [J]. Pharmacol Res, 2005; 52(1): 14.
15. Decker P, Muller S. Modulating poly (ADPribose) polymerase activity: potential for the prevention and therapy of pathogenic situations involving DNA damage and oxidative stress [J]. Curr Pharm Biotechnol, 2002; 3(3): 275283.
16. Mazzon E, Genovese T, Di Paola R, et al. Effects of 3aminobenzamide, an inhibitor of poly (ADPribose) polymerase, in a mouse model of acute pancreatitis induced by cerulein [J]. Eur J Pharmacol, 2006; 549(13): 149156.
17. Du L, Zhang X, Han YY, et al. Intramitochondrial poly(ADPribosylation) contributes to NAD+ depletion and cell death induced by oxidative stress [J]. J Biol Chem, 2003; 278(20): 1842618433.
18. Pandol SJ, Saluja AK, Imrie CW, et al. Acute pancreatitis: bench to the bedside [J]. Gastroenterology, 2007; 133(3): 1056.e11056.e25.
19. Gukovskaya AS, Pandol SJ. Cell death pathways in pancreatitis and pancreatic cancer [J]. Pancreatology, 2004; 4(6): 567586.
20. Nakajima H, Nagaso H, Kakui N, et al. Critical role of the automodification of poly (ADPribose) polymerase1 in nuclear factorkappaBdependent gene expression in primary cultured mouse glial cells [J]. J Biol Chem, 2004; 279(41): 4277442786.
21. Szabó C, Ischiropoulos H, Radi R. Peroxynitrite: biochemistry, pathophysiology and development of therapeutics [J]. Nat Rev Drug Discov, 2007; 6(8): 662680.
22. Pacher P, Szabo C. Role of the peroxynitritepoly (ADPribose) polymerase pathway in human disease [J]. Am J Pathol, 2008; 173(1): 213.
23. Mota RA, SánchezBueno F, Saenz L, et al. Inhibition of poly (ADPribose) polymerase attenuates the severity of acute pancreatitis and associated lung injury [J]. Lab Invest, 2005; 85(10): 12501262.
24. Ku Y, Sharif R, Dhaulakhandi D, et al. Saluja A.Inhibition of poly (ADP ribose) polymerase attenuates the severity of caeruleininduced pancreatitis and associated lung injury by inhibition of NFkB activation [J]. Pancreas, 2003; 27(4): 392.
25. Hashimoto K, Ethridge RT, Saito H, et al. The PPARgamma ligand, 15dPGJ2, attenuates the severity of ceruleininduced acute pancreatitis [J]. Pancreas, 2003; 27(1): 5866.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Significance of mRNA Expression of PARP-1 in Severe Acute Pancreatitis Associated Renal Injury in Rats

Abstract Full text Figures/Tables Video References Cited by

Format

Content