Objective To investigate the expression of RNA editase ADAR1 in the lymphocytes in rats’ spleen with liver transplantation rejection.
Methods Thirty SD rats and 75 Wistar rats were included. Fifteen livers from Wistar rats were transplanted to 15 Wistar rats (isograft group), 30 livers from SD rats were transplanted to 30 Wistar rats (allograft group and allograft+FK506 group), and 15 of them were then intramuscularly injected with FK506, 2 mg/(kg·d), the other 15 Wistar rats were only operated similarly to the other rats without any liver transplantation (control group). Five rats were killed and their splenetic tissues were collected on day 3, day 5, and day 7, respectively. The expression of ADAR1 mRNA in lymphocytes of the spleen in acute rejection was detected by RT-PCR.
Results Different performance of pathology was observed in all the liver and spleen tissues from the transplanted rats over time, especially in allograft group. The expression of ADAR1 mRNA in the allograft group was significant higher than that of isograft and allograft+FK506 groups （P＜0.001）, especially on the 5th day.
Conclusion There was a significant positive correlation between expression of ADAR1 and the severity of acute rejection, but the mechanism by which ADAR1 affected the acute rejection is unknown and needs to be further studied. FK506 may inhibit the expression of ADAR1 and remarkably reduce the severity of acute rejection.
Citation：
ZHANG Hongtao,DOU Kefeng,LI Jun,FENG Quanxin,KONG Yalin,ZHANG Fuqin,ZHAO Qingchuan. ADAR1 Expression in Acute Rejection of Liver Transplantation in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(2): 134-137. doi:
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| 1. |
Peng Y, Gong JP, Yan LN, et al. Improved twocuff technique for orthotopic liver transplantation in rat [J].Hepatobiliary Pancreat Dis Int, 2004; 3(1)∶33.
|
| 2. |
Lefkowitch JH. Diagnostic issues in liver transplantation pathology [J]. Clin Liver Dis, 2002; 6(2)∶555.
|
| 3. |
于颖彦， 沈柏用， 周光文， 等. Banff方案对临床肝移植后急性排斥反应分级的指导作用 [J]. 中华器官移植杂志， 2003； 24（2）∶76.
|
| 4. |
Athanasiadis A, Rich A, Maas S. Widespread AtoI RNA editing of Alucontaining mRNAs in the human transcriptome [J]. PLoS Biol, 2004; 2(12)∶e391.
|
| 5. |
罗晓星， 杨静华. 全组织提取物检测AtoI RNA编辑酶活性 [J]. 生物技术， 2002； 12（3）∶122.
|
| 6. |
罗晓星， 杨静华， Rabinovici R. 内毒素对小鼠多种组织中A至I RNA编辑酶活性和mRNA中肌苷的诱导 [J]. 科学通报， 2002； 47（20）∶1556.
|
| 7. |
Yang JH, Luo X, Nie Y, et al. Widespread inosinecontaining mRNA in lymphocytes regulated by ADAR1 in response to inflammation [J]. Immunology, 2003; 109(1)∶15.
|
| 8. |
赵青川， 张德新， 罗晓星， 等. 小鼠原代淋巴细胞增殖过程中RNA编辑酶ADAR1的变化 [J]. 细胞与分子免疫学杂志， 2002； 18（3）∶208.
|
| 9. |
孔亚林， 赵青川， 冯全新， 等. ADAR1在共培养体系中表达的初步研究 [J]. 第四军医大学学报, 2006； 27（5）∶392.
|
| 10. |
孔亚林， 窦科峰， 冯全新， 等. RNA编辑酶ADAR1在小鼠细胞免疫应答中的作用研究 [J]. 中国普外基础与临床杂志, 2006; 13（3）∶279.
|
| 11. |
Nie Y, Ding L, Kao PN, et al. ADAR1 interacts with NF90 through doublestranded RNA and regulates NF90mediated gene expression independently of RNA editing [J]. Mol Cell Biol, 2005; 25(16)∶6956.
|
- 1. Peng Y, Gong JP, Yan LN, et al. Improved twocuff technique for orthotopic liver transplantation in rat [J].Hepatobiliary Pancreat Dis Int, 2004; 3(1)∶33.
- 2. Lefkowitch JH. Diagnostic issues in liver transplantation pathology [J]. Clin Liver Dis, 2002; 6(2)∶555.
- 3. 于颖彦， 沈柏用， 周光文， 等. Banff方案对临床肝移植后急性排斥反应分级的指导作用 [J]. 中华器官移植杂志， 2003； 24（2）∶76.
- 4. Athanasiadis A, Rich A, Maas S. Widespread AtoI RNA editing of Alucontaining mRNAs in the human transcriptome [J]. PLoS Biol, 2004; 2(12)∶e391.
- 5. 罗晓星， 杨静华. 全组织提取物检测AtoI RNA编辑酶活性 [J]. 生物技术， 2002； 12（3）∶122.
- 6. 罗晓星， 杨静华， Rabinovici R. 内毒素对小鼠多种组织中A至I RNA编辑酶活性和mRNA中肌苷的诱导 [J]. 科学通报， 2002； 47（20）∶1556.
- 7. Yang JH, Luo X, Nie Y, et al. Widespread inosinecontaining mRNA in lymphocytes regulated by ADAR1 in response to inflammation [J]. Immunology, 2003; 109(1)∶15.
- 8. 赵青川， 张德新， 罗晓星， 等. 小鼠原代淋巴细胞增殖过程中RNA编辑酶ADAR1的变化 [J]. 细胞与分子免疫学杂志， 2002； 18（3）∶208.
- 9. 孔亚林， 赵青川， 冯全新， 等. ADAR1在共培养体系中表达的初步研究 [J]. 第四军医大学学报, 2006； 27（5）∶392.
- 10. 孔亚林， 窦科峰， 冯全新， 等. RNA编辑酶ADAR1在小鼠细胞免疫应答中的作用研究 [J]. 中国普外基础与临床杂志, 2006; 13（3）∶279.
- 11. Nie Y, Ding L, Kao PN, et al. ADAR1 interacts with NF90 through doublestranded RNA and regulates NF90mediated gene expression independently of RNA editing [J]. Mol Cell Biol, 2005; 25(16)∶6956.
