Objective To investigate the expression changes of nuclear factor kappa B (NF-κB) and matrix metalloproteinase-9 (MMP-9) in the cultured hepatocellular carcinoma cells 9204 (HCC9204) transfected with inhibitory kappa B alpha（IκB-α)vector.
Methods After pcDNA3-IκB-α vector and pcDNA3 were transfected into HCC9204 by lipofectamine method, Western-blot and RT-PCR analysis were used to detect the expressions of NF-κB and MMP-9. Migration and invasion of tumor cells were assayed by fundus membrane invaded by them.
Results When pcDNA3-IκB-α was transfected into HCC9204, the expression of NF-κB was decreased at the protein level, and the expression of MMP-9 mRNA and the invision and metastasis ability of transfected cells were obviously decreased.
Conclusion When the activity of NF-κB is inhibited, the ability of invasion and metastasis in HCC9204 cells decrease, which could be related to the decreased the expression of MMP-9.
Citation：
ZHANG Kejun,GAO Yanming,YANG Jinyong,ZHANG Bingyuan. Effect of Hepatocellular Carcinoma Cells Transfected with Inhibitory Kappa B Alpha on Expression of Nuclear Factor Kappa B and Matrix Metalloproteinase-9. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(2): 185-187. doi:
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张克君， 高焱明. 核因子-κB p65在肝炎相关性肝细胞癌中的表达及意义 [Ｊ]. 中国癌症杂志， 2005； 15（1）∶12.
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- 1. Shishodia S, Aggarwal BB. Nuclear factor-kappa B activation mediates cellular transformation, proliferation, invasion angiogenesis and metastasis of cancer [Ｊ]. Cancer Treat Res， 2004; 119∶139.
- 2. Shukla S, MacLennan GT, Fu P, et al. Nuclear factor-kappa B/p65 (Rel A) is constitutively activated in human prostate adenocarcinoma and correlates with disease progression [Ｊ]. Neoplasia， 2004; 6(4)∶390.
- 3. Andela VB, Schwarz EM, Puzas JE, et al. Tumor metastasis and the reciprocal regulation of prometastatic and antimetastatic factors by nuclear factor kappa B [Ｊ]. Cancer Res， 2000; 60(23)∶6557.
- 4. Jiang Y, Cui L, Yie TA, et al. Inhibition of anchorage-independent growth and lung metastasis of A549 lung carcinoma cells by I kappa B beta [Ｊ]. Oncogene， 2001; 20(18)∶2254.
- 5. Hah N, Lee ST. An absolute role of the PKC-dependent NF-kappa B activation for induction of MMP-9 in hepatocellular carcinoma cells [Ｊ]. Biochem Biophys Res Commun， 2003; 305(2)∶428.
- 6. Ortega N, Jonca F, Vincent S, et al. Systemic activation of the vascular endothelial growth factor receptor KDR/flk-1 selectively triggers endothelial cells with an angiogenic phenotype [Ｊ]. Am J Pathol, 1997; 151(5)∶1215.
- 7. Li Z, Ren Y, Wu QC, et al. Macrophage migration inhibitory factor enhances neoplastic cell invasion by inducing the expression of matrix metalloproteinase 9 and interleukin-8 in nasopharyngeal carcinoma cell lines [Ｊ]. Chin Med J (Engl), 2004; 117(1)∶107.
- 8. 张克君， 高焱明， 杨堂斗， 等. 核因子-κB p65、IκB-α在肝炎相关性肝细胞癌中的表达及临床意义 [Ｊ]. 实用肿瘤杂志， 2004; 19(3)∶216.
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