Effect of Hypoxia on Expression of VEGF in HepG2 Cells_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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LI Xingrui , LIAO Xiaofeng , YI Jilin.

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Keywords：

Hepatocellular carcinoma Vascular endothelial growth factor Transcatheterized hepatic arterial chemoembolization

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【Abstract】Objective To investigate the change of vascular endothelial growth factor (VEGF) expression in HepG2 cells under hypoxia.
Methods HepG2 cells were cultured under hypoxia（hypoxia group） and normal condition (control group). VEGF expression of HepG2 cells was examined by immunohistochemical staining. The growth of HepG2 cells was examined by MTT colorimetry and cell count. VEGF level in the culture medium was measured by ELISA.
Results After 48 h and 72 h of culture, the growth rate of HepG2 cells in hypoxia group was lower than that in control group (P＜0.05). The cell count in hypoxia group (2.51×104/μl and 2.69×104/μl, respectively) was much lower than that in control group(3.01×104/μl and 3.52×104/μl) after 48h and 72h of culture (P＜0.05). In hypoxia group, VEGF level in the culture medium after 24 h and 48 h was higher than that in control group (P＜0.05, P＜0.01).
Conclusion Hypoxia may enhance the VEGF expression in HepG2 cells and this could be the reason of high expression of VEGF after transcatheterized hepatic arterial chemoembolization.

Citation： LI Xingrui,LIAO Xiaofeng,YI Jilin.. Effect of Hypoxia on Expression of VEGF in HepG2 Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(5): 477-479. doi: Copy

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1. Huang J, He X, Lin X, et al. Effect of preoperative transcatheter arterial chemoembolization on tumor cell activity in hepatocellular carcinoma [Ｊ]. Chin Med J (Engl), 2000; 113(5)∶446.
2. 廖晓峰,易继林,杨志芳，等. 肝动脉化疗栓塞对肝癌组织血管内皮生长因子表达的影响 [Ｊ]. 肿瘤研究与临床， 2001； 13(6)∶383.
3. Cahill BA, Braccia D. Current treatment for hepatocellular carcinoma [Ｊ]. Clin J Oncol Nurs, 2004; 8(4)∶393.
4. Befeler AS, Di Bisceglie AM. Hepatocellular carcinoma: diagnosis and treatment [Ｊ]. Gastroenterology, 2002; 122(6)∶1609.
5. 廖晓峰,　易继林,　杨志芳，等. 肝细胞癌栓塞化疗后肿瘤微血管密度的变化 [Ｊ]. 华中科技大学学报(医学版)， 2002； 31(5)∶544.
6. Kleespies A, Guba M, Jauch KW, et al. Vascular endothelial growth factor in esophageal cancer [Ｊ]. J Surg Oncol, 2004; 87(2)∶95.
7. von Marschall Z, Cramer T, Hocker M, et al. Dual mechanism of vascular endothelial growth factor upregulation by hypoxia in human hepatocellular carcinoma [Ｊ]. Gut, 2001; 48(1)∶87.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Hypoxia on Expression of VEGF in HepG2 Cells

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