EXPERIMENTAL STUDIES OF TRANSPLANTATION OF ENDOTHELIAL CELLS DERIVED FROM HUMAN SAPHANOUS VEINS ONTO BLOOD VESSEL PROSTHESES_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of General Surgery, Zhujian Hospital, The First Military Medical University, Guangzhou 510282;

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Endothelium Transplantation Blood vessel prosthesis

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Objective To study the feasibility of transplanting human saphanous vein endothelial cells to luminal surface of blood vessel prosthesis and to play a theoretical foundation for the clinical application of autologous endothelial cell transplantation.
Methods Human saphanous vein endothelial cells were harvested with 0.1% collagenase and cultivated in vitro for 13.08±1.24 days. The cultures were confirmed as endothelial cells with the fourescent linked anti-Ⅷ antigen antibodies. The content of both 6-keto-PGF1α and Von Willebrand factor (vWF) in the supernatant were detected with ELISA and radioimmunoassay. The multiplied cells were lined in vitro onto the luminal surface of expanded polytetraflouroethylene (ePTFE) grafts precoated with fibrin glue and fibronectin, then cultivated again for 9 days.
Results 11.46±2.69×１０６ of available endothelial cells could be regularly obtained, the number of endothelial cells increased 147.93±88.68 times when culture were terminated. All the cells diploid cells with a purity of 99%. The content of both 6-keto-PGF1α and vWF in the media showed no significant difference between the primary and subculture passages. The luminal surface of grafts was covered completely by a spindlelike endothelial monolayer and an even fibrin glue matrix could be seen underneath.
Conclusion Endothelial cells derived from human saphanous veins might be feasible to be transplanted onto the luminal surface of ePTFE and present a potential clinical application.

Citation： PAN Yuxian,ZHENG Zhenhua,HE Hongbing,et al.. EXPERIMENTAL STUDIES OF TRANSPLANTATION OF ENDOTHELIAL CELLS DERIVED FROM HUMAN SAPHANOUS VEINS ONTO BLOOD VESSEL PROSTHESES. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(1): 13-15. doi: Copy

1.	Zilla P, Fasol R, Dudeck U, et al. In site cannulation microgrid follow up and lowdensity plating provide first passage endothelial cell masscultures for in vitro lining 〔Ｊ〕. J Vasc Surg, 1999; 12(2)∶180.
2.	Williams SK, Kleinert LB, Rose D, et al. Origin of endothelial cells that line expanded polytetrafluorethylene vascular grafts sodded with cells from microvacularized fat 〔Ｊ〕. J Vasc Surg, 1994; 19(4)∶594.
3.	Visser MJ, Van Bockel JH, Van Muijen GN, et al. Cells derived from omental fat tissue and used for seeding vascular prostheses are not endothelial in origin: a study on the origin of epitheloid cells derived from omentum 〔Ｊ〕. J Vasc Surg, 1991; 13(3)∶373.
4.	Potzsch B, Grulich Henn J, Rossing R, et al. Identification of endothelial and mesothelial cells in human omental tissue and in omentumderived cultured cells by specific cell markers 〔Ｊ〕. Lab Invest, 1990; 63(6)∶841.
5.	Fischlein T, Zilla P, Meinhart J, et al. In vitro endothelialization of amesosystemic shunt: a clinical case report 〔Ｊ〕. J Vasc Surg, 1994; 19(3)∶549.

1. Zilla P, Fasol R, Dudeck U, et al. In site cannulation microgrid follow up and lowdensity plating provide first passage endothelial cell masscultures for in vitro lining 〔Ｊ〕. J Vasc Surg, 1999; 12(2)∶180.
2. Williams SK, Kleinert LB, Rose D, et al. Origin of endothelial cells that line expanded polytetrafluorethylene vascular grafts sodded with cells from microvacularized fat 〔Ｊ〕. J Vasc Surg, 1994; 19(4)∶594.
3. Visser MJ, Van Bockel JH, Van Muijen GN, et al. Cells derived from omental fat tissue and used for seeding vascular prostheses are not endothelial in origin: a study on the origin of epitheloid cells derived from omentum 〔Ｊ〕. J Vasc Surg, 1991; 13(3)∶373.
4. Potzsch B, Grulich Henn J, Rossing R, et al. Identification of endothelial and mesothelial cells in human omental tissue and in omentumderived cultured cells by specific cell markers 〔Ｊ〕. Lab Invest, 1990; 63(6)∶841.
5. Fischlein T, Zilla P, Meinhart J, et al. In vitro endothelialization of amesosystemic shunt: a clinical case report 〔Ｊ〕. J Vasc Surg, 1994; 19(3)∶549.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

EXPERIMENTAL STUDIES OF TRANSPLANTATION OF ENDOTHELIAL CELLS DERIVED FROM HUMAN SAPHANOUS VEINS ONTO BLOOD VESSEL PROSTHESES

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