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find Author "汪曾炜" 21 results
  • 离体兔肺缺血再灌注损伤肺表面活性蛋白变化及保护

    目的 探讨最佳肺泡内氧浓度及温度对肺缺血再灌注损伤的保护作用.方法 离体兔肺在不同肺泡内氧浓度及不同温度下用非氧合血行肺动脉灌注30分钟,测定支气管肺泡灌洗液中血浆白蛋白(ALB),肺表面活性蛋白(SP,SP-A,SP-B,SP-C)占总蛋白含量的百分比,PS的活性并进行组织学观察.结果 肺泡内氧浓度与SP含量及活性成正比,与ALB含量成反比(Plt;0.01);肺泡内温度与ALB含量成正比,与SP含量及活性成反比(Plt;0.01).结论 (1)离体兔肺损伤明显,并减低了SP的含量和活性;(2)支气管肺泡灌洗液中SP,ALB含量和PS活性的测定是评价离体肺缺血再灌注损伤程度的可靠指标;(3)肺泡内充氧和低温对离体兔肺SP合成、活性、肺泡Ⅱ型细胞结构和功能有较好的保护作用.

    Release date:2016-08-30 06:35 Export PDF Favorites Scan
  • 严重左心室功能不全冠心病患者的外科治疗

    目的总结有严重左心室功能不全[左心射血分数(LVEF)≤0.35]冠状动脉粥样硬化性心脏病(冠心病)患者行冠状动脉旁路移植术(CABG)的临床经验。方法18例有严重左心室功能不全的冠心病患者冠状动脉造影均显示为3支血管病变,在体外循环下行CABG,采用左乳内动脉18支与前降支吻合,采用桡动脉11支及大隐静脉26支与其它血管吻合。所有患者术前、术后均行正电子发射断层18F-脱氧葡萄糖显像(18F-FDGPET)检查,以判定心肌的存活状况。结果手术死亡1例,死于心室颤动。12例使用主动脉内球囊反搏(IABP),术后二次气管内插管3例。随访17例,随访时间14~26个月,所有患者心功能较术前均有不同程度的改善,LVEF(0.51±0.13)较术前(≤0.35)增大。2例出院后出现心绞痛复发。结论CABG是治疗严重左心室功能不全冠心病患者的有效治疗方法,其效果取决于存活心肌的多少及可再血管化的目标血管的数量。术中良好的心肌保护,积极应用IABP及护心通是手术成功的关键。

    Release date:2016-08-30 06:25 Export PDF Favorites Scan
  • The Long Effect of Nonpulsatile Flow on Changes of Structure and Function in Pulmonary Microcirculation

    Objective To investigate the long effect of nonpulsatile flow on changes of structure and function in pulmonary microcirculation and to identify the pulmonary reconstruction under this blood perfusion. Methods Canine models with nonpulsatile flow in the right lung was established, and sacrificed 6 months later. Compare endothelial nitric oxide synthase (eNOS) in vascular endothelium, apoptosis in smooth muscle cell with immunohistochemistry by streptavidinbioepidermmultienzyme complex methodes, and observe structural changes in pulmonary arterioles with optical microscope. Results The expression of eNOS in the right nonpulsatile flow perfusing lung was weaker as compared to the left lung (10 846.7±177.8 vs. 13 136.1±189.6;t=2.240, P=0.040), the fas was ber as compared to the left lung(14 254.1±217.1 vs. 11 976.7±195.7; t=2.160, P=0.040). The ratio of wall thichness/vessel diameter in the right lung(13.64%±12.80% vs. 14.96%±13.10%) and wall area/vessel area(46.40%±11.70% vs. 47.80%±12.20%) was lower as compared to the left lung(Plt;0.05). Conclusion Longterm nonpulsatile flow can decrease the expression of eNOS, contract the muscles in capillary net, and increase pulmonary vascular resistance. Moreover it canincrease the arteriole apoptosis, leading to vascular structure remodeling.

    Release date:2016-08-30 06:05 Export PDF Favorites Scan
  • 心脏肿瘤的外科治疗

    目的总结心脏肿瘤的发生部位、肿瘤性质、诊断和外科治疗方法。方法1979年1月至2003年6月对195例心脏肿瘤患者行手术治疗,其中良性肿瘤186例(95.38%)、恶性肿瘤9例(4.62%);完整切除肿瘤188例,大部分或部分切除肿瘤7例。结果手术死亡5例,死亡率2.56%(5/195);手术成功190例(97.44%),术后随访3个月至15年,随访率96.84%(184/190),6例粘液瘤患者于术后4个月~2年内复发,4例恶性肿瘤分别于术后5个月~2年内死亡。结论心脏肿瘤以粘液瘤多见,彻底切除肿瘤是防止心脏肿瘤复发的关键,心脏恶性肿瘤手术近远期效果差;近10年心脏肿瘤呈逐年增加的趋势。

    Release date:2016-08-30 06:25 Export PDF Favorites Scan
  • Surgical treatment for double outlet of ventricle with atrioventricular discordance

    Objective To report the surgical treatment for double outlet of ventricle with atrioventricular discordance. Methods 11 patients aged from 3 to 25 years underwent surgical treatment for double outlet of ventricle with atrioventricular discordance. 10 of them were double outlet right ventricle and the other one was double outlet left ventricle. The surgical treatment included biventricular repair (n=9) and single ventricular repair (n=2). The biventricular repair was performed by intraventricular patch repair and extracardiac valved conduit or homograft valved conduit. The single ventricular repair included bidirected Glenn operation and total cavopulmonary connect. Results There were two early deaths in biventricular repair and no death in single ventricular repair. The cause of death was severe low cardiac output syndrome. Four survivors in biventricular repair were followed up from 1 to 11.5 years, three of them were in NYHA class Ⅰ or Ⅱ and one in NYHA class Ⅲ. Conclusions Double outlet of ventricle with atrioventricular discordance can be treated by biventricular repair or single ventricular repair according to the development of ventricle and pulmonary arteries. Closure of ventricular septal defect , no obstruction from ventricle to great artery and no harm of conduction system are the keys of conventional management to achieve good results.

    Release date:2016-08-30 06:27 Export PDF Favorites Scan
  • 老年瓣膜病患者心瓣膜置换术的临床分析

    目的 总结老年瓣膜病患者行心瓣膜置换术的经验,以提高手术效果。 方法 回顾性分析2000年1月至2009年4月沈阳军区总医院收治74例老年瓣膜病患者行心瓣膜置换术的临床资料,其中男51例,女23例;年龄60~74岁。术前经心电图、胸部X线片、彩色超声心动图、主动脉根部造影和左心室选择性造影检查诊断,均经手术证实,单纯二尖瓣病变45例;单纯主动脉瓣病变13例,其中7例为先天性二叶主动脉瓣畸形导致退行性改变;主动脉瓣和二尖瓣联合病变16例。均在全身麻醉体外循环下行心瓣膜置换术。 结果 全组无手术死亡,围术期死亡2例。74例患者体外循环时间58.0~136.5 min,主动脉阻断时间36.0~102.0 min。生存患者均无明显并发症,术后9~32 d顺利出院。 结论 心瓣膜置换术是治疗老年瓣膜病的安全方法,对左心房增大者进行减容并对三尖瓣关闭不全者进行良好处理,可获得满意的临床效果。

    Release date:2016-08-30 05:57 Export PDF Favorites Scan
  • Therapeutic efficacy of extracardiac conduit total cavopulmonary connection

    Objective To summary the experience of extracardiac conduit total cavopulmonary connection (TCPC) and study the operative indication, design, method, and therapeutic efficacy. Methods 29 patients of extracardiac conduit TCPC were reviewed:the average age was 10 years. Of them, there were 9 cases of tricuspid atresia, 9 double inlet ventricle with left ventricular type, 3 mitral atresia, 3 corrected transposition of the great arteries with anatomically right ventricular hypoplasia and 5 double outlet of right ventricle with left ventricular hypoplasia. All patients underwent cardiopulmonary bypass, 12 cases with heart arrested, and 17 without heart arrested. In them, 20 cases’ superior vena cava were anastomosed directly to the upper margin of right pulmonary artery, 9 cases deviated to the left side of right pulmonary artery to enlarge the stoma. For the inferior vena cava stoma, 22 cases’ anterior walls of right atrium were partially incised, and sutured to the posterior wall, then anastomosed with Gore-Tex blood vessel prostheses and connected to pulmonary trunk, and the other 7 cases’ bottom of right atrium was totally incised, the proximal was closed, and the distal was anastomosised with Gore-Tex blood vessel prostheses and connected to the lower margin of right pulmonary artery, deviated to the right sidedness. Results 5 died in the first 22 cases, and the next 7 cases all survive. All patients were followed up for 3 months to 10 years with no late death. Of them, 12 cases had low cardiac output syndrome, and 11 cases of chylothorax. Conclusions Compared with other types of Fontan operation, the extracardiac conduit TCPC has better long-term effects in older or grown-up children. Nevertheless, strict operative adoption, reasonable operative design, refined procedures, carefully observation and treatment are the key points of improving therapeutic efficacy.

    Release date:2016-08-30 06:27 Export PDF Favorites Scan
  • Experimental Study on the Isolation, Culture and Identification of Mesenchymal Stem Cells from Human Bone Marrow in Vitro

    Objective To observe the morphology and growing status of mesenchymal stem cells(MSCs) of human bone marrow in vitro, in order to confirm that MSCs of human bone marrow are ideal seed cells and provide basic theory for further MSCs research. Methods The methods of density gradient centrifugation with lymphocyte separation medium for human and adherent filtration were used to isolate and purify MSCs of human bone marrow. We observed the cellular growth status and morphology of the primary MSCs and the surface antigens of second passage MSCs were tested. Results The primary culture cells fused into monolayer after 14-16 d. The passage cells kept the same morphological characteristics of primary culture cells. Ultrastructure of the second passage MSCs showed that the shape of nuclei was irregular, there were multiple nucleoli in some of the nuclei, and morphological differentiation of intracytoplasm organelles was immature. The growth curve of the first, fifth and tenth passage cells showed a logarithmic growth at day 3, a peak growth at day 5, and no clones occurred after tenth passage. Cloning efficiency of first passage, fifth passage and tenth passage was respectively 25.83%±2.93%, 14.67%±1.63% and 4.67%±0.52%. Test of MSCs phenotypic characteristics showed a high homogeneity among the cells and surface antigen profiles were positive for CD29, CD44 and negative for CD34, CD45. Conclusion The methods of density gradient centrifugation with lymphocyte separation medium for human and adherent filtration are simple, economic and efficient to isolate and purify MSCs from human bone marrow. With a high proliferating ability in vitro, MSCs from human bone marrow are ideal seed cells for tissue engineers.

    Release date:2016-08-30 05:59 Export PDF Favorites Scan
  • Surgical Technique and Outcomes for Supracardiac Total Anomalous Pulmonary Venous Connection

    Objective To summarize the surgical experience of supracardiac total anomalous pulmonary venous connection(S-TAPVC) and study the surgical technique and outcomes for S -TAPVC. Methods Eightysix patients with S-TAPVC underwent the surgical repair from May 1985 to December 2007. There were 49 males and 37 females. The patients aged from 7 months to 35 years (mean 9.6 years) and weighed from 4.9 kg to 68.0 kg (mean 23.8 kg). The patients were divided into three groups by the approach for the anastomosis. There were 20 patients in groupⅠthrough the right atrium incision, 49 patients in group Ⅱ through the right and left atrium incisions and 17 patients in group Ⅲ through the top of the left atrium incision. The interrupt continuous anastomosis between the common pulmonary venous and the left atrium was used in all patients. The enlarged atrial septal defect(ASD) was repaired with autopericardium. The vertical vein was ligated if the postoperative left atrial pressure was less than 15 mm Hg. But the vertical vein was opened or just partialy ligated if the postoperative left atrial pressure was more than 15 mm Hg. Results There was no early operative death. The postoperative left atrial pressure in three groups were 9.3±3.2 mm Hg, 9.9±2.9 mm Hg and 11.6±3.8 mm Hg, respectively. The cases with open or just partly ligated vertical vein in three groups were 0 case (0%), 7 cases (14.3%) and 2 cases (11.8%), respectively. The cases of arrhythmia in three groups were 5 cases (25.0%), 15 cases (30.6%)and 1 case (5.9%). The severely low cardiac output syndrome occurred in 2 patients and reoperation for bleeding in 2 patients. The morbidity of arrhythmia in group Ⅲ was less than in group Ⅱ(P=0.042). Conclusion The outcome of surgical repair for S -TAPVC is satisfactory. The anastomosis through the top of the left atrium incision has low occurrence of arrhythmia. The anastomosis through the right and left atrium incision is easy to expose and to perform surgery, especial for old children and adult patients.

    Release date:2016-08-30 06:05 Export PDF Favorites Scan
  • 埃布斯坦综合征合并预激综合征的外科治疗

    目的 为了提高手术疗效,总结埃布斯坦综合征(Ebstein syndrome)合并预激综合征的手术治疗经验. 方法 11例患者均有埃布斯坦综合征及严重三尖瓣关闭不全,合并A型预激综合征4例,B型 7例.术中电生理标测显示11例中有12条附加旁道,右心室游离壁6例,左心室游离壁3例,后间隔1例,后间隔和右心室游离壁双旁道1例.所有患者的房室附加旁道均在体外循环下被成功地手术切割;埃布斯坦综合征按Danielson方法行成形术9例,三尖瓣置换术2例. 结果 全组11例无手术后早、晚期死亡;术后随访4个月~6年,平均随访3.4±1.3年,无预激综合征复发,2例有轻度三尖瓣关闭不全.心功能Ⅰ级9例,Ⅱ级2例. 结论 手术治疗埃布斯坦综合征合并预激综合征,可同时纠正埃布斯坦综合征伴三尖瓣关闭不全和预激综合征,治疗效果良好.

    Release date:2016-08-30 06:32 Export PDF Favorites Scan
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