ObjectiveTo investigate the effect of dust fine particles on tumor necrosis factor-α (TNF-α), matrix metalloproteinase (MMP), transforming growth factor-β1 (TGF-β1), and collagens in the lung tissue of rats.MethodsAccording to random number table method, 96 male Wistar rats were divided into an untreated control group, a treated control group and an experimental group, with 32 rats in each group. The experimental group was exposed to the wind tunnel simulation of sandstorm (5 days per week, 5 hours per day); the untreated control group was put in the standard living environment next to the wind tunnel; the treated control group was exposed to the same wind tunnel simulation of sandstorm for 5 hours every day, the speed of wind was the same as the experimental group, but without dust; On the 30th, 60th, 90th, and 120th day, the levels of TNF-α, MMP-2, MMP-9, TGF-β1, lung collagen type Ⅰ and Ⅲ in the lung tissue of rats were determined by enzyme linked immunosorbent assay.ResultsCompared with the untreated control group and the treated control group, the content of TNF-α was higher in the experimental group on 30th, 60th, 90th and 120th day (all P<0.05). The contents of MMP-9 and MMP-2 in the experimental group on 60th and 90th day were significantly higher than those in the untreated group and the treated control group, respectively (all P<0.05). On the 30th, 60th, 90th, and 120th day, the content of TGF-β1 in the experimental group was significantly higher compared with the two control groups (all P<0.05). The contents of lung collagen type Ⅰ and type Ⅲ were higher in the experimental group on 60th, 90th and 120th day, respectively, compared with the two control groups (all P<0.05).ConclusionsThe strong sandstorm environmental exposure to a certain period of time can promote lung interstitial collagen deposition in rat. With the prolonged exposure time, the deposition of collagen increases. TNF-α, MMP-2, MMP-9 and TGF-β1 may all participate and induce the process of pulmonary fibrosis.
Tuberculosis is a chronic infectious diseases caused by Mycobacterium tuberculosis. Its high morbidity and mortality have posed a serious threat to global public health. Matrix metalloproteinase (MMP) is a proteolytic enzyme involved in regulating extracellular matrix degradation and remodeling. MMP is highly expressed in pulmonary tuberculosis, and its expression is regulated by genes, epigenetic modifications, cellular signaling pathways, immune regulation, and cellular environment. MMP is a potential target for the treatment of pulmonary tuberculosis. Therefore, this article summarizes the expression and related mechanisms of MMP in pulmonary tuberculosis, aiming to provide a reference for the diagnosis and treatment of pulmonary tuberculosis.
ObjectiveTo explore the present state of research, emerging trends, and key topics in the field of liquid biopsy for lung cancer, offering insights for the holistic management of the disease. MethodsData was sourced from the Web of Science Core Collection database, focusing on literature related to liquid biopsy in lung cancer published between 2012 and 2025. Tools such as CiteSpace and Biblioshiny were employed to perform a detailed visual analysis of various aspects, including publication outputs, contributing countries and institutions, international collaborations, leading authors, prominent journals, academic disciplines, keyword distributions, and cited references. ResultsA total of 1 128 articles were analyzed. Findings indicated that research in the area of liquid biopsy for lung cancer experienced rapid growth since 2014, peaking in 2022. The majority of research efforts were centered in China and the United States. The French institution Institut National de la Sante et de la Recherche Medicale (INSERM) leaded in publication output. Malapelle U was the most prolific author. The journal Cancers published the highest number of related articles. Keywords analysis highlighted liquid biopsy and lung cancer as central research themes. Key research topics consistently included circulating tumor DNA, circulating tumor cells, extracellular vesicles, epidermal growth factor receptor mutations, and DNA methylation in the context of liquid biopsy. Meanwhile, immunotherapy and minimal residual disease emerged as frontier areas in this domain. ConclusionThe bibliometric results demonstrate a continuous rise in scholarly output on liquid biopsy in lung cancer. The identified research hotspots and evolving trends offer valuable guidance for future studies, with the ultimate goal of facilitating broader clinical adoption of liquid biopsy technologies and advancing precision medicine in lung cancer treatment.